Part:BBa_K4799990:Design
pAtMRP3
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 915
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 915
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 915
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 915
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the expression of AtMRP3 increases after exposure to cadmium, I decided to add AtMRP3 before the luminescence gene. As a result, the gene's expression is controlled by cadmium concentration. Therefore, the light intensity of the plant can be used to indicate cadmium concentration in the soil.
Source
Arabidopsis thaliana
References
Zientara K, Wawrzyńska A, Łukomska J, López-Moya JR, Liszewska F, Assunção AGL et al. Activity of the AtMRP3 promoter in transgenic Arabidopsis thaliana and Nicotiana tabacum plants is increased by cadmium, nickel, arsenic, cobalt and lead but not by zinc and iron. Journal of Biotechnology. 2009;139(3):258-263. https://doi.org/10.1016/j.jbiotec.2008.12.001
BOVET, L., EGGMANN, T., MEYLAN-BETTEX, M., POLIER, J., KAMMER, P., MARIN, E., FELLER, U. and MARTINOIA, E. (2003), Transcript levels of AtMRPs after cadmium treatment: induction of AtMRP3. Plant, Cell & Environment, 26: 371-381. https://doi.org/10.1046/j.1365-3040.2003.00968.x