Regulatory

Part:BBa_K4799990:Design

Designed by: Youran Yao, Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-07)


pAtMRP3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 915
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 915
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 915
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 915
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the expression of AtMRP3 increases after exposure to cadmium, I decided to add AtMRP3 before the luminescence gene. As a result, the gene's expression is controlled by cadmium concentration. Therefore, the light intensity of the plant can be used to indicate cadmium concentration in the soil.


Source

Arabidopsis thaliana

References

Zientara K, Wawrzyńska A, Łukomska J, López-Moya JR, Liszewska F, Assunção AGL et al. Activity of the AtMRP3 promoter in transgenic Arabidopsis thaliana and Nicotiana tabacum plants is increased by cadmium, nickel, arsenic, cobalt and lead but not by zinc and iron. Journal of Biotechnology. 2009;139(3):258-263. https://doi.org/10.1016/j.jbiotec.2008.12.001

BOVET, L., EGGMANN, T., MEYLAN-BETTEX, M., POLIER, J., KAMMER, P., MARIN, E., FELLER, U. and MARTINOIA, E. (2003), Transcript levels of AtMRPs after cadmium treatment: induction of AtMRP3. Plant, Cell & Environment, 26: 371-381. https://doi.org/10.1046/j.1365-3040.2003.00968.x